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Funding limitations prevented Nyren from dedicating much time to the project at first, but in 1993, he was finally able to publish a proof-of-principle. His technique began by mixing the template DNA with a primer, a single dNTP, and three enzymes: the familiar DNA polymerase plus the light cascade enzymes, ATP sulfurylase and firefly luciferase.6 If the dNTP was incorporated into a strand of DNA, PPi would be produced in the chemical reaction. ATP sulfurylase could then convert the PPi into ATP, which would provide energy for the luciferase enzyme, producing light. Thus, it was possible to determine each base in the sequence by cycling through the dNTPs one at a time until light was detected, and then washing extra nucleotides out between each step. By literally rinsing and repeating, the sequence could be recorded one letter at a time without the use of any gels, which often took hours to run and were difficult to automate.
新篇章已然开启，中国人保正大步向前。“十五五”时期，中国人保将坚持和加强党的全面领导，持之以恒推进全面从严治党，筑牢高质量发展的“根”与“魂”。通过持续强化政治建设和能力建设，将党建优势转化为治理效能和发展势能，锻造一支政治过硬、能力过硬的队伍，确保公司始终沿着正确的方向前进。，这一点在PDF资料中也有详细论述
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